The phrase at least two different courses means that we need to inspect two tuples belonging to the same student.
Therefore, two renamed copies of $\mathrm{Marks}$ are required.
First, they must refer to the same student:
$\mathrm{M_1.studentID=M_2.studentID}$.
Both tuples must correspond to $\mathrm{CPSC}$ courses:
$\mathrm{M_1.courseType}=\mathrm{'CPSC'}$ and $\mathrm{M_2.courseType}=\mathrm{'CPSC'}$.
Finally, they must represent different courses:
$\mathrm{M_1.courseID\neq M_2.courseID}$.
Thus A expresses exactly the required condition.
In B, requiring the same $\mathrm{courseID}$ allows a tuple to match itself, which does not prove that the student took two distinct courses.
C only proves that a student took at least one $\mathrm{CPSC}$ course.
D finds different courses but does not require them to belong to the same student.
Therefore, the correct answer is A.